Spectroscopic and Thermodynamic Characterization of Human 3-Hydroxyanthranilate-3,4-Dioxygenase

Different kynurenine pathway enzymes limit quinolinic acid formation by various human cell types.

Substantial increases in the tryptophan-kynurenine pathway metabolites, l-kynurenine and the neurotoxin quinolinic acid, occur in human brain, blood and systemic tissues during immune activation. Studies in vitro have shown that not all human cells are capable of synthesizing quinolinate. To investigate further the mechanisms that limit l-kynurenine and quinolinate production, the activities of...

Crystal structures of human 3-hydroxyanthranilate 3,4-dioxygenase with native and non-native metals bound in the active site.

3-Hydroxyanthranilate 3,4-dioxygenase (3HAO) is an enzyme in the microglial branch of the kynurenine pathway of tryptophan degradation. 3HAO is a non-heme iron-containing, ring-cleaving extradiol dioxygenase that catalyzes the addition of both atoms of O2 to the kynurenine pathway metabolite 3-hydroxyanthranilic acid (3-HANA) to form quinolinic acid (QUIN). QUIN is a highly potent excitotoxin t...

0993. Heme oxygenase - 1 attenuates acute pulmonary inflammation by decreasing the release of segmented neutrophils from the bone marrow

BPDO biphenyl dioxygenase BphC 2,3-dihydroxybiphenyl 1,2-dioxygenase CPO chloroperoxidase CPR cytochrome P450 oxidoreductase HbpA 2-hydroxybiphenyl 3-monooxygenase NDO naphthalene dioxygenase PAH polycyclic aromatic hydrocarbon PCB polychlorinated biphenyl pNCA p-nitrophenoxy-carboxylic acid TDO toluene dioxygenase TOM toluene ortho-monooxygenase

Oxygenase enzymes have seen limited practical applications because of their complexity, poor stabilities, and often low catalytic rates. However, their ability to perform difficult chemistry with high selectivity and specificity has kept oxygenases at the forefront of engineering efforts. Growing understanding of structure–function relationships and improved protein engineering methods are pavi...

Correlation of LDL Cholesterol with Maternal and Cord Blood Heme Oxygenase 1 in Preeclampsia

Emerging evidence supports an important role for the heme oxygenase system (HO-1) in the maintenance of a healthy pregnancy, especially during pathological challenge. HO-1 is widely accepted to be a highly sensitive and reliable marker of oxidative stress. Hence the present study was planned to analyse heme oxygenase-1 and lipid profile in maternal and cord blood venous samples of normal pregna...